Figure 3. Cytotoxicity and clonogenic activity of erufosine in Rb-deficient cells.

The figure depicts the survival (A) or clonogenicity (B) of SKW-3 cell clones with 99% (shRNA1) or 83% (shRNA 2) stable Rb-knockdown after treatment with 2, 4, 8, 16 or 32 µM erufosine for 48 h. A significant difference versus the respective nonsense control is marked by an asterisk (Student’s t-test; p<0.05). Bars denote standard deviation. The table under the graphs gives the IC₅₀ values of erufosine after 48 h of treatment as well as the respective 95% confidence intervals.