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. 2014 Jul 2;9(7):e100950. doi: 10.1371/journal.pone.0100950

Figure 6. Altered expression levels of cell cycle regulators impair the cytotoxic activity of erufosine by Rb-knockdown.

Figure 6

The columns (A) present the expression of the transcription factor E2F2 on mRNA level in SKW-3 cell clones with 99% (shRNA 1) and 83% (shRNA 2) stable Rb-knockdown after treatment with 16 µM erufosine for 24 h. The values and standard errors are calculated after normalization according to the ratio target gene versus reference gene (GAPDH) as shown in the table beside the graphs. The expression of proteins related to the cell cycle regulation before and after treatment with 16 µM erufosine for 48 h is given in panel B. The values under the protein bands denote their intensity compared to the untreated nonsense control and are calculated after densitometric analysis with the Quantity One 4.6.6 Program (Bio-Rad).