Figure 5. B2G2 inhibits the activation of various transcription factors, decreases survivin expression, and induces cleaved PARP expression in different human PCa cell lines.

PC3, 22Rv1 and C4-2B cells were cultured as described in “Materials and methods”, and treated with either DMSO alone (control) or varying concentrations of B2G2. At the end of the treatments, both adherent and non-adherent cells were harvested and nuclear extracts/total cell lysates were prepared. A and B) Using nuclear extracts EMSA was performed for NF-κB and AP1 followed by drying of gels and autoradiography as detailed in “Materials and methods”. C and D) Nuclear extracts/total cell lysates were subjected to SDS-PAGE followed by Western immunoblotting. Membranes were probed with pStat3 (tyr 705), total Stat3, survivin and cleaved PARP antibodies followed by peroxidase-conjugated appropriate secondary antibody and visualized by ECL detection system. Equal protein loading was confirmed by stripping and re-probing the membranes with β-actin. C, Control; B2G2, procyanidin B2 3,3″-di-O-gallate.