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. 2014 Jul 2;9(7):e100685. doi: 10.1371/journal.pone.0100685

Figure 7. Effects of triptolide and SFN on Nrf2-related downstream targets in livers of BALB/C mice.

Figure 7

(A) The mRNA levels of NQO1, GCLC and HO-1 were analyzed by real-time PCR. GAPDH was used as an internal control. The data are represented as the mean ± SD; n = 6; *P<0.05, **P<0.01 vs Con group. (B) The protein levels of NQO1, GCLC and HO-1 were analyzed by Western blot. β-actin was used as an internal control. A representative blot from three independent experiments is shown. The density of the immunoreactive bands was analyzed, and the data are represented as the means ± SD; n = 6; *P<0.05, **P<0.01 versus Con group.