Figure 1. H₂O₂ induces DNA DSB signaling in WT and uPAR−/− mouse VSMC.

VSMC isolated from WT (A) and uPAR−/− (B) mice were treated with 100 µM H₂O₂ for indicated time. Phosphorylation of ATM, Chk-2 and H2AX was assessed by western blotting. C. H₂O₂-induced phosphorylation of ATM, Chk-2 and H2AX was quantified from 3 independent experiments. Data are shown as folds of increase relative to unstimulated control and normalized to the total level of corresponding protein.