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. 2014 Jul 2;9(7):e101662. doi: 10.1371/journal.pone.0101662

Figure 3. Kinetics of 2-HED mediated inactivation of AnGDH forward activity.

Figure 3

AnGDH (50 µg pure protein in 200 µl) was treated with different concentrations of 2-HED (•, 0 mM; ○, 0.5 mM; ▾, 1.0 mM; Δ, 2.0 mM; ▪, 3.0 mM and □, 4.0 mM). Aliquots (20 µl) were withdrawn (and diluted suitably) at time points indicated, to monitor the residual activity by the standard assay. Inset: log-log plot of kapp versus 2-HED concentrations (with corresponding symbols) to obtain the number of catalytically important thiols modified.