Table 2. Effect of different disulfides on forward and reverse activities of AnGDH.
| Disulfide (5.0 mM) | Residual activity (%) | |
| Forward | Reverse | |
| 2-HED | 0.8±0.2 | 100.4±5.8 |
| GSSG | 100.7±7.3 | 100.0±2.3 |
| Cystine (saturateda) | 8.3±1.9 | 101.0±4.8 |
a
Saturating concentration in water corresponds to 0.47 mM at 25°C [56].
AnGDH (1.0 U) was incubated for 1 h with each disulfide at 37°C and the residual activity was measured under standard assay conditions. The AnGDH activity remaining without any disulfide treatment after 1 h incubation is taken as control (100%). Mean ± standard deviation (n≥2) are presented.