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. 2014 May 30;12:151. doi: 10.1186/1479-5876-12-151

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Copyright © 2014 Quandt et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Significant increase of B7-H1 surface expression by IL-4 and TNFα treatment of RCC cell lines. Different RCC cell lines (Hal31RC, Hal161RC, MZ1790RC) were treated with either IL-4 or TNFα alone before B7-H expression was determined by flow cytometry as described in Materials and Methods at 72 hrs. A) Shown are FACS histogram overlay plots for B7-H1, B7-H2, B7-H3 and B7-H4. Each plot shows overlay graphs, depicting constitutive protein expression and expression upon single or combined cytokine treatment. Representative data of at least 3 different experiments are shown. B) Cytokine-mediated induction of B7-H1 and B7-H2 in % using constitutive expression levels as reference value. Combined data of 3 different experiments are given.