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. 2014 Jul 2;9(7):e101530. doi: 10.1371/journal.pone.0101530

Figure 4. TRAF6 and IRAK1 decrease hypoxia/reoxygenation-induced inflammatory responses in macrophages.

Figure 4

RAW264.7 cells were transfected with three siRNAs targeting IRAK1 or TRAF6 for 24(A and B) qRT-PCR analysis of IRAK1 and TRAF6. (C) Immunoblotting of IRAK1 and TRAF6. (D) Immunoblotting of IRAK1 and TRAF6 after transfected with the effective siRNA, negative control for 24 h. RAW264.7 cells were transfected with siRNA, negative control in presence of miR-146a inhibitor or its control for 24 h, and followed hypoxia/reoxygenation. (E and F) ELISA of TNF-α and IL-6 in supermatants. Results are mean ± SD. *, P<0.05 and **,P<0.01,***,P<0.001 versus negative control. I1, IRAK1; T6, TRAF6; NC, negative control siRNA; I, miR-146a inhibitor; iNC, negative control miRNA of miR-146a inhibitor.