Figure 6. Spot assay of E. coli growth.

The viabilities of the strains were detected on different LB plated with additional 500/L NaCl (B), 800 mmol/L NaCl and normal LB plates (A). Strains were Control: strain harboring plasmid pET-28(a), pET-ALDHs: strain harboring different SiALDH proteins. 1.5 microliters of the serially diluted E. coli cells cultured for 16 h at 16°C with 0.5 mmol/L IPTG were dotted onto LB agar plates containing NaCl at the marked concentration.