Skip to main content
. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cell Metab. 2014 Jun 12;20(1):61–72. doi: 10.1016/j.cmet.2014.05.004

Figure 4. Glut1 is required for activation-induced metabolic reprogramming.

Figure 4

(A, B) Control (Glut1fl/fl) and LckCreGlut1fl/fl T cells were rested in IL-7 or CD3/CD28-stimulated and (A) glucose uptake or (B) glycolytic rate was measured after 16h. (C-F) Control (Glut1fl/fl) and CD4CreGlut1fl/fl T cells were rested in IL-7 or CD3/CD28-stimulated for 16h. (C) Total lactate produced was measured. (D) Extracellular acidification rate (ECAR) was assessed after the addition of glucose (gluc), oligomycin (oligo), and 2-deoxyglucose (2-DG) at indicated times and (E) glycolytic capacity and (F) Oxygen Consumption Rate (OCR)/ECAR ratio determined. Mean ± SD (n=4) are shown from a minimum of 2 or more independent experiments.