A) Gene expression analysis of proximal colon in untreated, and in response to 3 DSS treatments, in male RID tg and wildtype mice. (B) Spleen, (C) fasting serum-insulin and (D) SAA1 and 2 mRNA levels in liver in control or antibiotic-treated male RID tg and wildtype mice. 1-way ANOVA analysis shows that both treatment (F=15.8/8.7/12.1/5.8, p=0.001/0.003/0.004/0.03) and genotype (F=19.3/17.1/23.6/31.8, p<0.001/0.001/<0.001/<0.001) contribute significantly to spleen size, insulin levels, liver SAA1 and liver SAA2 levels. (E) Summary and proposed model: Acute inflammation is essential for healthy adipose tissue expansion and proper remodeling. Inability of adipose tissue to accurately sense and respond to inflammatory stimuli leads to reduced adipose tissue expansion and an increased risk for microbial translocation. Error bars represent SEM, a p-value <0.05 according to student t-test was considered as significant and is indicated by */#; **/##: p<0.01; ***: p<0.001 (*significantly different from WT, # significantly different from untreated controls of same genotype).