Figure 2. Characterization of liver specific Raptor knockout mice.

(A) (Left panel) Raptor PCR on genomic DNA extracted from tail, whole liver and isolated hepatocytes of WT, Raptor^F/+ (F/+), Raptor^F/F (F/F or FF), and Raptor^Δhep (KO) mice. (Right panel) Immunoblot analysis of isolated hepatocytes from F/F and KO mice with raptor, p70S6K, and its phosphorylation antibody. (B) Serum ALT and liver weight as percentage of body weight (LW/BW) in 8 weeks old FF and KO mice (n= 4). (C, D) H&E, Sirius red, and immune cell staining of liver sections from FF and KO mice (scale bar: 100 μm). Sirius red positive areas were quantified with Image J software and shown as bar graphs (n= 4). Immune cell infiltration was analyzed by immunostaining of liver sections with indicated antibodies. Bar graphs indicate frequencies of positive cells (n= 4). (E, F) TUNEL, cleaved-caspase 3 (clv-casp 3), and phospho-γH2AX staining were performed on liver sections, positive cells were quantified and results are shown in the bar graphs (n= 4). All the bar graphs in Figure 2 represent means +/− S.D. See also Figure S2.