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. 2014 Jun 18;45(1):66. doi: 10.1186/1297-9716-45-66

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Copyright © 2014 Wei et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of different functional domains of Muscovy duck MDA5 on the chicken IFN-β promoter. The pCAGGS-MDA5, pCAGGS-MDA5ΔRD, pCAGGS-MDA5ΔCARD, pCAGGS-MDA5ΔCARD + ΔRD, pCAGGS-MDA5-CARD or the empty plasmid was cotransfected with pGL3-chIFNβ-Luc and pTK-RL plasmid in DEF cells. Dual-luciferase assays were performed after 36 h.