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Effects of the CARD of Muscovy duck MDA5 on the chicken IRF-7 and NF-κB promoters. The DEF cells were cotransfected with pGL3-chIRF-7-Luc (or pGL3-chNF-κB-Luc) and the pTK-RL plasmid and with pCAGGS-MDA5-CARD or with the empty plasmid. Dual-luciferase assays were performed after 36 h.
