Table 1.
Gene cloning PCR primers used in this study
| Primer name | Sequence of Oligonucleotide (5′ → 3′) | Purpose |
|---|---|---|
| idMDA5-f |
AGRSMTTACCARATGGAAGTKG |
Gene cloning |
| idMDA5-r |
AARTGTTCTGCACARACRCGWTC |
|
| idPKR-f |
CACCTAATTTTGATAATGCAAGAAA |
Gene cloning |
| idPKR-r |
ATAAATGTCTACTTCCTTTCCATA |
|
| idOASL-f |
TTCCTCAAGGAGCGCTGCTTC |
Gene cloning |
| idOASL-r |
GGGTCGGCGGGATCCAGGAT |
|
| 5rdM5r-1 |
AATGAGATTTTCAGCTGAGAATCACCAC |
5′-Race |
| 5rdM5r-2 |
TGATCTTTGGTAATGTAAACAG |
|
| 3rdM5f-1 |
GATCTCAGCCATATGAACAGTGGGTG |
3′-Race |
| 3rdM5f-2 |
TGATGATGATGATGAACCAGC |
|
| dMDA5-f |
ATGTCGACGGAGTGCCGAGACG |
Site mutation |
| dMDA5-site mutation-r |
AGGTGCTCTCATCAGCACGAGCTCGAC |
|
| dMDA5-site mutation-f |
GCCCGTGGTCGAGCTCGTGCTGATGAG |
Site mutation |
| dMDA5-r |
TCAGTCTTCATCACTTGAAGGACA |
|
| pCAGGS-MDA5-f |
TAACTCGAGACCATGTCGACGGAGTGCCGAGACG |
MDA5 cloning |
| pCAGGS-MDA5-r |
ATTGCTAGCTCACTTGTCATCGTCGTCCTTGTAGTCATCTTCATCACTTGA |
|
| pCAGGS-MDA5ΔRD-f |
TAACTCGAGACCATGTCGACGGAGTGCCGAGACG |
MDA5-ΔRD cloning |
| pCAGGS-MDA5ΔRD-r |
ATTGCTAGCTCACTTGTCATCGTCGTCCTTGTAGTCAGGGTTTTTCTTATATG |
|
| pCAGGS-MDA5ΔCARD-f |
TAACTCGAGACCATGACAGGAGGAAAAGAGAATAA |
MDA5-ΔCARD cloning |
| pCAGGS-MDA5ΔCARD-r |
ATTGCTAGCTCACTTGTCATCGTCGTCCTTGTAGTCATCTTCATCACTTGA |
|
| pCAGGS-MDA5ΔCARD + ΔRD-f |
TAACTCGAGACCATGACAGGAGGAAAAGAGAATAA |
MDA5ΔCARD + ΔRD cloning |
| pCAGGS-MDA5ΔCARD + ΔRD-r |
ATTGCTAGCTCACTTGTCATCGTCGTCCTTGTAGTCAGGGTTTTTCTTATATG |
|
| pCAGGS-MDA5-CARD -f |
TAACTCGAGACCATGTCGACGGAGTGCCGAGACG |
MDA5-CARD cloning |
| pCAGGS-MDA5-CARD -r |
ATTGCTAGCTCACTTGTCATCGTCGTCCTTGTAGTCATTTCCACTTAAATCAT |
|
| pCAGGS-DK212-NS1-f |
TAACTCGAGACCATGGATTCCAACACTGT |
DK212-NS1 cloning |
| pCAGGS-DK212-NS1-r | ATTGCTAGCTCACTTGTCATCGTCGTCCTTGTAGTCAACTTTTGACTCAAT |
Note: CGTCTC was the recognition site of restriction enzyme Xho I and GCTAGC was the recognition site of restriction enzyme Nhe I. R = A/G, S = C/G, M = A/C, K = G/T, W = A/T. f = forward primer; r = reverse primer.