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Measurement of the moving distance on a treadmill. Treadmill has a metal bar that provides a foot shock (0.4 mA micro current) that causes a tingling sensation. After rats were subjected to a running test on a treadmill at 15 cm/sec, the moving distance on a metal bar were measured during three-times of foot shock. Data are presented as mean±SEM. A value of P<0.05 (*) was considered to be statistically significant compared to control.
