Fig. 6.

M-3-stimulated glucose uptake is PI3K-dependent and mimicked by sAPPα. Basal and insulin-stimulated 2DG uptake in vehicle and M-3-treated myotubes in the absence (a) and presence (b) of wortmannin (Wm; n = 6–12). (c) Gain in cell surface GLUT4myc in control and M-3- and insulin-treated myotubes ± wortmannin (n = 4–11). Representative immunoblots of sAPPα- (d) and sAPPβ- (e) stimulated PKB phosphorylation at Ser⁴⁷³ and total PKB in myotubes. Histograms show normalised means ± SEM of immunoblots (n = 5–6). (f) 2DG uptake in myotubes treated with vehicle or sAPPα (n = 8). (g) Relative increase in 2DG uptake from vehicle, insulin and sAPPα (3 nmol/l) stimulated myotubes (n = 14–16). Veh, vehicle. *p < 0.05, **p < 0.01, ***p < 0.001 vs vehicle; ^† p < 0.05, ^†† p < 0.01, ^††† p < 0.001