Table 1.
Fatty acid compositions (percentage of total fatty acids) of total phospholipids of heart tissue from fish fed the reference (REF) and functional (CMS1 and CMS2) feeds at different times before (PreCh) and after (6, 8, 10, 12 and 14 weeks) infection with PMCV
| PreCh | 6 wpc | 8 wpc | 10 wpc | 12 wpc | 14 wpc | TWO WAY ANOVA P-value | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| REF | CMS1 | CMS2 | REF | CMS1 | CMS2 | REF | CMS1 | CMS2 | REF | CMS1 | CMS2 | REF | CMS1 | CMS2 | REF | CMS1 | CMS2 | Diet | Week | Diet*Week | |
| Saturated | 25.9 ± 0.8 | 31.2 ± 0.9 | 31.9 ± 0.8 | 27.6 ± 0.8 | 29.7 ± 0.7 | 30.1 ± 0.6 | 27.2 ± 0.6 | 29.9 ± 0.7 | 29.2 ± 0.5 | 27.1 ± 0.5 | 29.5 ± 0.3 | 29.9 ± 0.5 | 27.6 ± 1.5 | 30.6 ± 1.4 | 31.2 ± 1.0 | 27.9 ± 0.5 | 31.3 ± 0.4 | 30.4 ± 0.7 | 0.000 | 0.005 | ns |
| 18:1n-9 | 13.2 ± 0.6 | 7.4 ± 0.6 | 6.8 ± 0.3 | 9.5 ± 0.4 | 6.8 ± 0.2 | 7.2 ± 0.4 | 10.1 ± 0.3 | 7.2 ± 0.2 | 8.0 ± 0.4 | 10.2 ± 0.8 | 7.5 ± 0.6 | 7.5 ± 0.7 | 11.0 ± 1.5 | 7.3 ± 0.1 | 7.6 ± 0.3 | 11.5 ± 0.6 | 7.5 ± 0.2 | 7.3 ± 0.5 | 0.000 | 0.003 | 0.003 |
| Monounsat | 24.6 ± 2.6 | 15.4 ± 0.9 | 15.3 ± 0.7 | 18.0 ± 1.0 | 14.7 ± 0.8 | 15.1 ± 1.1 | 19.5 ± 0.7 | 14.9 ± 0.7 | 16.8 ± 0.9 | 18.9 ± 1.6 | 15.6 ± 1.3 | 15.8 ± 1.9 | 20.4 ± 3.0 | 15.5 ± 0.4 | 15.8 ± 0.7 | 21.5 ± 1.4 | 15.7 ± 0.6 | 15.5 ± 1.0 | 0.000 | 0.033 | 0.065 |
| 18:2n-6 | 4.3 ± 0.4 | 1.9 ± 0.1 | 1.7 ± 0.1 | 3.3 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.1 | 3.4 ± 0.1 | 1.8 ± 0.1 | 2.1 ± 0.2 | 3.3 ± 0.2 | 1.8 ± 0.2 | 1.8 ± 0.1 | 3.4 ± 0.4 | 1.7 ± 0.0 | 1.7 ± 0.1 | 3.5 ± 0.2 | 1.8 ± 0.1 | 1.8 ± 0.1 | 0.000 | 0.008 | 0.021 |
| 20:3n-6 | 0.6 ± 0.0 | 0.1 ± 0.0 | 0.2 ± 0.0 | 0.6 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 | 0.6 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.5 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.4 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.4 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 | 0.000 | 0.000 | 0.000 |
| 20:4n-6 | 1.4 ± 0.0 | 1.7 ± 0.1 | 1.6 ± 0.1 | 1.5 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.1 | 1.5 ± 0.0 | 1.7 ± 0.1 | 1.6 ± 0.0 | 1.4 ± 0.1 | 1.7 ± 0.0 | 1.7 ± 0.2 | 1.3 ± 0.1 | 1.8 ± 0.1 | 1.7 ± 0.1 | 1.4 ± 0.1 | 1.8 ± 0.1 | 1.8 ± 0.1 | 0.000 | 0.056 | 0.002 |
| n-6 PUFA | 7.3 ± 0.4 | 4.7 ± 0.2 | 4.5 ± 0.1 | 6.2 ± 0.1 | 4.5 ± 0.1 | 4.5 ± 0.1 | 6.2 ± 0.1 | 4.7 ± 0.0 | 4.8 ± 0.2 | 6.1 ± 0.2 | 4.8 ± 0.2 | 4.7 ± 0.1 | 6.0 ± 0.4 | 4.6 ± 0.1 | 4.6 ± 0.1 | 6.4 ± 0.2 | 5.0 ± 0.1 | 4.9 ± 0.1 | 0.000 | 0.000 | 0.000 |
| 18:3n-3 | 1.5 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.0 | 1.1 ± 0.0 | 0.4 ± 0.0 | 0.4 ± 0.0 | 1.2 ± 0.1 | 0.5 ± 0.0 | 0.6 ± 0.1 | 1.3 ± 0.1 | 0.5 ± 0.0 | 0.5 ± 0.0 | 1.3 ± 0.2 | 0.4 ± 0.0 | 0.4 ± 0.0 | 1.4 ± 0.0 | 0.5 ± 0.0 | 0.5 ± 0.0 | 0.000 | 0.000 | 0.001 |
| 20:5n-3 | 6.3 ± 0.3 | 9.7 ± 0.3 | 9.6 ± 0.4 | 7.7 ± 0.3 | 10.6 ± 0.3 | 10.4 ± 0.3 | 8.0 ± 0.3 | 10.5 ± 0.2 | 10.2 ± 0.3 | 8.4 ± 0.4 | 11.3 ± 0.4 | 10.8 ± 0.4 | 8.0 ± 0.1 | 10.9 ± 0.6 | 10.4 ± 0.2 | 7.7 ± 0.3 | 10.3 ± 0.4 | 11.0 ± 0.6 | 0.000 | 0.000 | 0.038 |
| 22:6n-3 | 31.5 ± 1.4 | 33.8 ± 1.3 | 33.9 ± 1.5 | 35.8 ± 1.4 | 35.3 ± 0.9 | 34.8 ± 1.5 | 34.6 ± 0.7 | 34.8 ± 0.5 | 33.6 ± 0.7 | 34.5 ± 0.8 | 33.2 ± 1.9 | 33.6 ± 1.5 | 33.4 ± 2.7 | 32.9 ± 1.2 | 32.8 ± 1.4 | 31.7 ± 1.5 | 32.4 ± 0.5 | 32.7 ± 1.0 | ns | 0.000 | ns |
| n-3 PUFA | 42.0 ± 1.4 | 48.3 ± 1.7 | 48.0 ± 1.8 | 47.4 ± 1.2 | 50.7 ± 0.8 | 50.0 ± 1.6 | 46.8 ± 0.8 | 50.3 ± 0.8 | 48.8 ± 0.8 | 47.3 ± 1.2 | 49.8 ± 1.7 | 49.4 ± 1.8 | 45.7 ± 2.5 | 48.9 ± 1.8 | 48.1 ± 1.5 | 43.8 ± 1.6 | 47.5 ± 0.5 | 48.8 ± 1.6 | 0.000 | 0.000 | ns |
| PUFA | 49.3 ± 0.8 | 53.0 ± 0.9 | 52.6 ± 0.7 | 53.7 ± 1.1 | 55.2 ± 0.8 | 54.5 ± 1.6 | 53.0 ± 0.8 | 55.0 ± 0.8 | 53.6 ± 0.6 | 53.4 ± 1.0 | 54.6 ± 1.5 | 54.0 ± 1.8 | 51.7 ± 2.2 | 53.5 ± 1.7 | 52.6 ± 1.5 | 50.2 ± 1.5 | 52.5 ± 0.6 | 53.7 ± 1.5 | 0.000 | 0.000 | ns |
| EPA/ARA | 4.6 ± 0.2 | 5.8 ± 0.4 | 5.8 ± 0.3 | 5.2 ± 0.2 | 6.3 ± 0.2 | 6.3 ± 0.3 | 5.5 ± 0.2 | 6.1 ± 0.0 | 6.5 ± 0.3 | 6.0 ± 0.2 | 6.6 ± 0.1 | 6.6 ± 0.4 | 6.1 ± 0.5 | 6.2 ± 0.3 | 6.3 ± 0.1 | 5.7 ± 0.1 | 5.7 ± 0.4 | 6.0 ± 0.3 | 0.000 | 0.000 | 0.001 |
| n-3/n-6 | 5.7 ± 0.2 | 10.3 ± 0.5 | 10.6 ± 0.3 | 7.6 ± 0.3 | 11.2 ± 0.3 | 11.2 ± 0.5 | 7.5 ± 0.2 | 10.6 ± 0.2 | 10.1 ± 0.5 | 7.7 ± 0.4 | 10.4 ± 0.8 | 10.6 ± 0.3 | 7.7 ± 1.0 | 10.6 ± 0.6 | 10.5 ± 0.3 | 6.9 ± 0.4 | 9.6 ± 0.1 | 10.1 ± 0.5 | 0.000 | 0.000 | 0.066 |
| Total lipid | 2.8 ± 0.3 | 3.5 ± 0.2 | 3.6 ± 0.2 | 3.32 ± 0.2 | 3.34 ± 0.2 | 3.49 ± 0.2 | 3.8 ± 0.4 | 3.34 ± 0.2 | 3.63 ± 0.6 | 3.84 ± 0.3 | 3.55 ± 0.2 | 3.58 ± 0.3 | 3.85 ± 0.2 | 3.56 ± 0.1 | 3.46 ± 0.1 | 3.81 ± 0.3 | 3.44 ± 0.5 | 3.46 ± 0.4 | ns | ns | ns |
ARA, arachidonic acid; DHA, docosahexaenoic acid (DHA); EPA, eicosapentaenoic acid; PUFA, polyunsaturated fatty acid.
Diet*Week indicates the p value of the interaction of those two factors in the 2-way ANOVA statistical analysis.