Table 2.
Folate concentration in HepG2 cells cultured in media containing different concentrations of pyridoxal
| Media Vitamin B-6 |
||||
|---|---|---|---|---|
| pmol/106 cells | 25 | 35 | 65 | 2,015 |
| THF | 12.7 ± 2.73a | 7.87 ± 0.99b | 8.10 ± 1.56b | 7.88 ± 0.6b |
| 5-Methyl-THF | 3.27 ± 0.67 | 2.62 ± 0.50 | 3.57 ± 0.56 | 3.48 ± 0.12 |
| 5,10-Methenyl-THF | 0.60 ± 0.31a | 0.32 ± 0.13a | 1.91 ± 0.54b | 2.44 ± 0.39b |
| Total folate | 16.6 ± 3.32a | 10.8 ± 1.20b | 13.6 ± 2.17a,b | 13.8 ± 0.49a,b |
Values are means + SD (nmol/l); n = 5. Different superscripts indicate significant difference between groups. In this analysis, both existing 5,10-methylene-THF and THF are quantified as THF (tetrahydrofolate), while existing cellular 5,10-methenyl-THF and 10-formyl-THF are measured as 5,10-methenyl-THF. P < 0.05 (one-way ANOVA). Within each row, values with a common superscript letter were not significantly different.