Table 3.
Intracellular transsulfuration metabolite concentrations in HepG2 cells cultured in media containing different vitamin B-6 concentrations
| Media Vitamin B-6 |
||||
|---|---|---|---|---|
| nmol/106 cells | 25 | 35 | 65 | 2,015 |
| Homocysteine | 0.012 ± 0.002a | 0.009 ± 0.002b | 0.003 ± 0.001c | 0.005 ± 0.001b,c |
| Glutathione | 3.76 ± 0.57a | 3.83 ± 1.09a | 1.26 ± 0.35b | 1.60 ± 0.28b |
| Cysteine | 1.18 ± 0.15a,c | 1.30 ± 0.23a | 0.88 ± 0.08b | 0.91 ± 0.14b,c |
| Cysteinylglycine | 0.31 ± 0.10a | 0.32 ± 0.07a | 0.03 ± 0.01b | 0.04 ± 0.01b |
Values are means ± SD (nmol/l); n = 5. Significance indicated at P < 0.05. Data were analyzed by one-way ANOVA. Within each row, values with a common superscript letter were not significantly different.