Fig. 1.

Effect of ANG II on (pro)renin receptor (PRR) mRNA and protein expression in primary rat inner medullary collecting duct (IMCD) cells. The cells were exposed to 1 μM ANG II at the indicated time periods and PRR expression was analyzed by immunoblotting and qRT-PCR. A: representative immunoblot of PRR. The full-length PRR protein was detected as a 43-kDa band. B: densitometric analysis of PRR protein. N = 3–6 per group. ^*P < 0.05 C: qRT-PCR analysis of PRR mRNA. The expression was normalized by GAPDH. N = 6 per group. Data are means ± SE.