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. 2014 Apr 16;307(1):F25–F32. doi: 10.1152/ajprenal.00548.2013

Fig. 3.

Fig. 3.

Effect of NS-398 on PRR expression in primary IMCD cells at baseline or after ANG II treatment. The cells were exposed to 1 μM ANG II for 12 h and PRR protein expression was analyzed by immunoblotting (A, B, and C). In a separate experiment, the cells were exposed to vehicle or NS-398 for 12 h and PRR was analyzed by the same method (D, E, and F). A, D: representative immunoblot of PRR. B, E: densitometric analysis of PRR protein. N = 6 per group. C, F: qRT-PCR analysis of PRR mRNA. D: expression was normalized by GAPDH. N = 6 per group. Data are means ± SE.