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Conditioned media from PGE2, transforming growth factor (TGF)-β, and PDGF-treated MPCs were used to assess tubule formation by IMCD cells grown in collagen I-3D gel cultures. A: IMCD3 cells treated with DMEM supplemented with PGE2. B: IMCD3 cells treated with CM from PGE2-treated MPCs show early tubule formation. C: IMCD3 cells treated with CM from TGF-β-treated MPCs do not show tubule formation. D: IMCD3 cells treated with CM from PDGF-treated MPCs do not show tubule formation.
