Fig. 9.
Conditioned medium from progenitor cells stimulates IMCD3 viability and proliferation. A: using an MTT assay for cell viability, the percentage of viable IMCD3 cells was calculated using absorbance of experimental group/absorbance of control group after 24 h of treatment with conditioned media. Treatment with conditioned media (CM) increased cell survival by nearly 100%. B: IMCD3 cells were treated for 48 h with progenitor cell CM. Progenitor cell nuclear antigen (PCNA) expression levels of treated IMCD3 cells were evaluated by Western blotting. PCNA levels increased in IMCD3 cells treated with 50 and 100% CM. *P < 0.005; n = 3.