Table 2.
Effects of overexpression of PNPLA3 with methionine (M148) or isoleucine (I148) at position 148 on the Huh-7 cells metabolome
| Main Pathway | Subpathway/Metabolite | Fold Change | Nominal P Value | Empirical P Value (FDR) |
|---|---|---|---|---|
| Overexpression of PNPLA3 with methionine at position 148 | ||||
| Amino acid metabolism | Tryptophan metabolism | |||
| Tryptophan | 0.8 | 0.002 | 0.06 | |
| Urea cycle | ||||
| Assymetricdimethylarginine (ADMA) | 0.6 | 0.009 | 0.05 | |
| Cysteine, methionine, SAM, taurine metabolism | ||||
| Methionine | 0.8 | 0.009 | 0.05 | |
| Valine, leucine, and isoleucine metabolism | ||||
| Valine | 0.8 | 0.009 | 0.06 | |
| Phenylalanine and tyrosine metabolism | ||||
| Phenylalanine | 0.8 | 0.009 | 0.06 | |
| Dipeptides | ||||
| Glycyl-isoleucine | 1.7 | 0.02 | 0.04 | |
| α-Amino Acids and Derivatives | ||||
| γ-Glutamyl-leucine | 1.6 | 0.03 | NS | |
| Lipid metabolism | Long-chain fatty acid | |||
| Docosatrienoate (22:3n3) | 0.8 | 0.002 | 0.02 | |
| Sterol/Steroid | ||||
| Cholesterol | 1.2 | 0.03 | NS | |
| Carbohydrate metabolism | Glycolysis, gluconeogenesis, pyruvate metabolism | |||
| Lactate | 1.8 | 0.002 | 0.02 | |
| Overexpression of PNPLA3 with isoleucine at position 148 | ||||
| Amino acid metabolism | α-Amino Acids and Derivatives | |||
| γ-Glutamyl-leucine | 1.9 | 0.006 | 0.007 | |
| Glutamate metabolism | ||||
| N-acetylglutamate | 1.3 | 0.06 | NS | |
| Lipid metabolism | Long-chain fatty acid | |||
| Docosatrienoate (22:3n3) | 0.8 | 0.02 | NS | |
| Sterol/Steroid | ||||
| Cholesterol | 1.3 | 0.03 | NS | |
| Nucleotides | Pyrimidine, cytidine metabolism | |||
| Cytidine | 0.8 | 0.02 | NS | |
Fold change refers to the ratio of the metabolite levels between M148 or Ile148 PNPLA3 expression vector-treated cells vs. empty vector- treated control cells, respectively. Nominal P value stands for test T after log transformation of variables with equal variances. Similar results were obtained by a nonparametric test.