Table 3.

Results of overrepresentation analysis: canonical pathways affected by differential regulation of metabolites during PNPLA3 silencing and overexpression of PNPLA3 with the I148M variants

Canonical Pathway	P Value
PNPLA3 silencing
Aminoacyl-tRNA biosynthesis	2.9 E−10
Glycerophospholipid metabolism	0.00002
Nitrogen metabolism	0.0002
Cyanoamino acid metabolism	0.005
Glycine, serine and threonine metabolism	0.02
Phenylalanine, tyrosine and tryptophan biosynthesis	0.02
Valine, leucine and isoleucine biosynthesis	0.02
d-Glutamine and d-glutamate metabolism	0.02
Arginine and proline metabolism	0.03
Pyrimidine metabolism	0.04
Overexpression of PNPLA3 with methionine at position 148 (M148)
Aminoacyl-tRNA biosynthesis	0.00003
Phenylalanine, tyrosine and tryptophan biosynthesis	0.002
Propanoate metabolism	0.004
Nitrogen metabolism	0.005
Overexpression of PNPLA3 with isoleucine at position 148 (I148)
Primary bile acid biosynthesis	0.06