Fig. 4.

Overexpression and knockdown of Sesn3 affect the AMPK signaling pathway in mice fed with ethanol. A: Western blotting of p-AMPK-α proteins in controls, AdGFP-, shGFP-, AdSesn3-, and shSesn3-injected mice. Actin was used as the loading controls. B and C: bar graphs represented the immunoblot analysis. Overexpression or knockdown Sesns3 also regulated expressions of genes encoding fatty acid synthesis (FAS) (D and E) and oxidation (F and G). ACC, acetyl-CoA carboxylase; PGC-1α, peroxisome proliferator-activated receptor-γ coactivator-1α; PPAR-α, peroxisome proliferator-activated receptor-α. *P < 0.05, significant difference compared with controls. ^{#,^}P < 0.05 vs. respective vector controls.