Fig. 5.

Overexpression and knockdown of Sesn3 affect the mTOR signaling and autophagy pathway in mice fed with ethanol. A and B: effect of ethanol on p70 S6K, a major downstream target of mTOR and marker of autophagy [light chain 3 (LC3)] in mice liver was analyzed by Western blotting. C and F: to confirm the findings in vivo, we performed autophagic flux assay using VL-17A cells, which were transfected with AdSesn3 and shSesn3 treated as indicated in the presence or absence of chloroquine (CQ; 25 μM). The immunoblot bands (C and D) were quantified by densitometry analysis, and the ratio of LC3-II (E) and p62 (F) to β-actin was calculated. *P < 0.05, significant difference compared with controls. ^$P < 0.05 vs. respective vector controls.