Fig. 3.
LPA/LPA5R acutely increase the Mf of apical NHE3 by a NHERF2- and LPA5R-dependent process. Transient expression of NHERF2 or LPA5R separately or together were examined for effects on NHE3 mobility. LPA (3 μM) fails to increase the NHE3 Mf in the apical domain of OK cells in the absence of either NHERF2 or LPA5R. Results are means ± SE of n > 4 replicates per condition. NHERF2/LPA5R-containing cells (2 bars at left) were used as a positive control for cells lacking either NHERF2 (2 bars at right) or LPA5R (2 bars at middle). P values compare LPA effects to control conditions lacking LPA.