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. 2014 Jul 3;4:5556. doi: 10.1038/srep05556

Figure 1. Flow cytometric analysis of Försters Resonance Energy Transfer (FRET) between cell surface LRP/LR and Aβ.

Figure 1

The fluorescence intensity histogram of the unlabelled non-permeabilised HEK293 cells (black histogram, a,c,e) was superimposed with that of cells labelled with the APC secondary antibody only (brown histogram, a,c,e) as well as with cells in which the proteins of interest (PrPc, CAT and Aβ42) were labelled with APC (a,c,e respectively). Co-labelling of LRP/LR-PE and PrPc-APC (positive control) (pink histogram, b). Co-labelling of LRP/LR-PE and CAT-APC (negative control) (green histogram, d). Co-labelling of LRP/LR-PE and Aβ-APC (red histogram, f). Each panel is a representative image. Three biological replicates, each performed in triplicate, were conducted.