Figure 2.

Fibrosis in laminin α2 chain-deficient muscle is associated with increased expression of miR-21. (A) Left: transverse sections of muscles from WT, dy^3K/dy^3K, and dy^2J/dy^2J mice stained for Fast green/Sirius red to visualize non-collagenous and collagenous tissue, respectively. Bar, 100 μm. Right: quantification of relative collagen content (fibrosis) in dy^3K/dy^3K and dy^2J/dy^2J mice (n ≥ 5). (B) RT-qPCR analysis of indicated miRNAs in muscles from dy^3K/dy^3K and dy^2J/dy^2J mice (n ≥ 4). (C) CD11b/CD68/Lamγ1 stained sections of muscles from WT, dy^3K/dy^3K, and dy^2J/dy^2J mice. Arrows indicate CD11b and CD68-positive cells (green). Bar, 100 μm. WT, wild-type; Lamγ1, laminin γ1 chain. Error bars represent SEM, ***P < 0.001.