Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Matrix Biol. 2014 Jan 26;34:46–54. doi: 10.1016/j.matbio.2013.12.011

Fig. 1.

Fig. 1

Vps34 is required for decorin evoked Peg3 induction in endothelial cells. [A] HUVEC were transfected with either scrambled siRNA (siScr, 20 pM) or Vps34-targeting siRNA (siVps34, 1 nM) and challenged with 200 nM decorin for 6 h in full HUVEC culture medium (please refer to Section 4.1 for additional details). Representative immunoblot analyses confirmed efficacy of Vps34 depletion. [B] HUVEC were transfected with siPeg3 (1 nM) and challenged with 200 nM decorin for 6 h relative to siScr (20 pM) transfected controls. Immunoblot confirmed RNAi-mediated silencing of Peg3 in HUVEC. GAPDH served as a positive loading control. Experiment was repeated at least four independent times with comparable results.