Fig. 2.

Decorin stimulates formation of a Vps34/Beclin 1 complex while simultaneously inhibiting formation of the Bcl-2/Beclin 1 complex. [A–C] Representative co-immunoprecipitation experiments performed in HUVEC with Beclin 1 [A], Vps34 [B], or VEGFR2 [C] immunoprecipitates following stimulation with 200 nM decorin for 6 h. All immunoprecipitates were executed with 5 µg of appropriate primary antibody. Notably, for Beclin 1 immunodetection [A,B], membranes were incubated with a mouse anti-rabbit light chain antibody following primary antibody incubation that effectively switches the antibody isotype from rabbit to mouse and thereby precludes detection of the heavy IgG chain in this region from immunoprecipitates. All experiments were repeated at least three independent times with similar results.