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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Matrix Biol. 2014 Jan 26;34:46–54. doi: 10.1016/j.matbio.2013.12.011

Fig. 3.

Fig. 3

Vps34 mobilizes from plasma membrane and co-localizes with Beclin 1 in a decorin-dependent manner. [A] Confocal laser scanning microscopy evaluating Vps34 and Beclin-1 distribution in HUVEC under basal conditions [B, left panel] Decorin (200 nM, 6 h) stimulated HUVEC depicting redistribution and co-localization of Vps34 with Beclin 1 as shown in the accompanying line scanning profile [B, right panel]. [C] HUVEC were pre-treated with Spautin-1 (10 µM, 30 min) then challenged with decorin (200 nM, 6 h) before evaluation of Vps34 and Beclin-1. Nuclei were stained with DAPI. Images were collected with the same gain, intensity, and exposure. Further, images are representative of multiple independent experiments (n = 4–5). Scale bar: ~6 µm.