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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Cell Rep. 2014 Jun 5;7(6):2019–2030. doi: 10.1016/j.celrep.2014.05.015

Figure 5. CCR7 surface downregulation requires Vpu’s transmembrane domain but not its conserved serines.

Figure 5

A) Primary CD4+ T cells were either mock-infected (i) or infected with DHIV (ii), DHIVΔVpu (iii) or DHIV-VpuS52,56N (iv), in which Vpu’s conserved serines were mutated to asparagines. All cells were surface stained for CCR7 expression followed by intracellular p24Gag staining, as in Figure 1B.

B) Primary CD4+ T cells were either mock-infected (i) or infected with DHIV (ii), or DHIV-VpuRD (iii), in which the transmembrane domain of Vpu was scrambled. Additionally, cells were infected with the indicated Vpu TM mutants (iv–x). Cells were stained and analyzed as described in A.