Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Cytometry A. 2014 Feb 22;85(5):386–399. doi: 10.1002/cyto.a.22452

Figure 2.

Figure 2

Effect of exposure of TK6 cells to different reported antiaging drugs on the level of constitutive expression of γH2AX. Exponentially growing TK6 cells were untreated (Ctrl) or treated with the respective agents for 24 h at concentrations as shown. Expression of γH2AX in individual cells was detected immunocytochemically with the phospho-specific Ab (AlexaFluor647; AF647), DNA was stained with DAPI; cellular fluorescence was measured by flow cytometry as described (72). Cells were gated in the respective phases of the cell cycle based on differences in DNA content, as marked by the dashed vertical lines. The percent decrease in mean γH2AX fluorescence intensity of the treated cells in particular phases of the cell cycle, with respect to the respective untreated controls, is shown above the arrows. Inserts present DNA content frequency histograms from the individual cultures. The dashed skewed lines show the background level, the mean fluorescence intensity of the cells stained with secondary Ab only (72).