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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Cytometry A. 2014 Feb 22;85(5):386–399. doi: 10.1002/cyto.a.22452

Figure 4.

Figure 4

The reduction of constitutive phosphorylation of ribosomal protein S6 (RP-S6) in TK6 cells treated with antiaging drugs. Exponentially growing TK6 cells were untreated (Ctrl) or treated with the respective agents at concentrations as shown, for 24 h. Based on differences in DNA content cells were gated in the respective phases of the cell cycle, as marked by the dashed vertical lines (Ctrl). The percent decrease in the RP-S6P mean fluorescence intensity of the treated cells in particular phases of the cell cycle, with respect to the same phases of the untreated cells, is shown above the arrows. The dashed skewed lines show the background level, the mean fluorescence intensity of the cells stained with secondary Ab only.