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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Cytometry A. 2014 Feb 22;85(5):386–399. doi: 10.1002/cyto.a.22452

Figure 6.

Figure 6

Decreased ratio of phosphorylated RP-S6 (RP-S6P) to total RP-S6 (RP-S6T) in A549 cells treated with different concentration of BRB. A549 cells were untreated (Ctrl) or treated for 24 h with BRB at concentration as shown, RP-S6P was then detected using phospho-specific primary Ab and secondary Ab tagged with AlexaFluor 647 (AF647) while total RP-S6 (RP-S6T) was detected using primary RP-S6 (not phospho-specific) Ab and secondary Ab AlexaFluor 488 (AF488), DNA was stained with DAPI. Intensity of cellular fluorescence was measured by laser scanning cytometry (LSC) and the ratiometric analysis of AF647/AF488 fluorescence intensities was carried out using the LSC software (128). The numbers on the left of the panels show the ratio of the mean values of integrated intensity of AF647 to AF488 fluorescence for all cells. The figures on right present the percent decrease of the ratio of BRB-treated cells vis-à-vis the Ctrl.