Table 2. Differential characteristics of strain H63T compared with its nearest neighbours based on 16S rRNA gene sequences.
Strains: 1, L. cardiaca sp. nov. H63T (data from this study); 2, L. brunensis ATCC 43878T (unless indicated, data from Wilkinson et al., 1988); 3, L. londiniensis ATCC 49505T (Dennis et al., 1993); 4, L. jordanis ATCC 33623T (Cherry et al., 1982); 5, L. erythra ATCC 35303T (Brenner et al., 1985), 6, L. dresdenensis DSM 19488T (Lück et al., 2010); 7, L. rubrilucens ATCC 35304T (Brenner et al., 1985); 8, L. feeleii ATCC 35072T (Brenner et al., 1985); 9, L. pneumophila ATCC 33152T (Brenner et al., 1979); 10, L. birminghamensis ATCC 43702T (Wilkinson et al., 1987). Reactions are scored as follows unless indicated: +, positive; +w, weakly positive; −, negative; ±, variable; nd, no data available. All strains grow on BCYE at 37 °C but do not grow under these conditions without cysteine, and all strains grow in BYE at 37 °C. All strains are positive for catalase and are negative for glucose fermentation, nitrate reduction and urease activity.
| Characteristics | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Pigment production when grown in BYE/BCYE at 37 °C | + | + | + | + | + | −* | + | +w | + | − |
| Autofluorescence when grown on BCYE at 37 °C | − | − | − | − | + | +w | + | − | − | + |
| Gelatinase | + | + | + | + | + | + | + | − | + | + |
| β-Lactamase | + | + | + | + | + | nd | + | − | + | + |
| Hippurate hydrolysis | + | − | −/+w | − | − | − | − | +w | + | − |
| Oxidase | +w | − | − | + | + | − | − | − | + | ± |
| Protease*† | + | + | +w | + | ++ | − | ++ | − | ++ | ++ |
| Phosphatase*† | + | ++ | − | +w | +w | +w | +w | − | ++ | − |
| Lipase*† | − | +w | +w | − | + | − | + | +w | ++ | +w |
| Swimming motility | + | −* | − | + | + | +* | + | + | + | + |
| Sliding motility* | − | − | − | − | − | − | − | + | + | − |
| Growth on BCYE at 17 °C*‡ | − | + | − | + | +++ | + | + | +++ | +++ | ++ |
| Growth on low-iron BCYE at 37 °C*‡ | + | ++ | + | + | +++ | +++ | +++ | +++ | +++ | +++ |
| Growth in deferrated CDM at 37 °C§ | + | ++ | +/− | +* | + | +* | +++ | +++ | +++ | ++ |
| Siderophore secretion§ | − | ++ | +/− | +* | +/− | −* | +++ | ++ | +++ | ++ |
| Salt sensitivity|| | s | s | r | r | s | s* | s | r | s | r |
Data from this study.
++, Strongly positive; +, positive; +w, weakly positive, slightly above background; −, negative.
Scored as follows in comparison with growth of spot dilutions on BCYE at 37 °C: +++, equal or 1 log less growth; ++, 2–4 logs less growth; +, 5–6 logs less growth; −, no growth.
Scored as follows: +++, high CDM growth/chrome azurol S (CAS) reactivity; ++, moderate CDM growth/CAS reactivity;+, slight CDM growth/CAS reactivity; +/−, CDM growth/CAS reactivity varied between experiments for unknown reasons (Starkenburg et al., 2004).
r, Resistant (similar level of growth on BCYE with or without NaCl); s, sensitive (reduced efficiency of plating on BCYE in the presence of 100 mM NaCl at 37 °C) (O’Connell et al., 1996).