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. 2014 Jun 12;12:166. doi: 10.1186/1479-5876-12-166

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Copyright © 2014 Sabnis et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Measurement of total and phosphorylated Akt 1/2/3 in AML cell lines. A) Electropherogram depicting levels of total Akt 1/2/3 in AML cell lines. AML cell lines MV4-11, MOLM-14, OCI-AML3 and HL60 were analyzed at baseline for activation of Akt. 80 ng of protein was used for analysis. β-2 Microglobulin was used as loading control. Total Akt antibody detects both phosphorylated and non-phosphorylated protein which is demonstrated on treatment with phosphatase. X-axis represents iso-electric pH and y-axis represents luminescence units. B) Different AML cell lines exhibit different levels of Akt phosphorylation as demonstrated using total Akt 1/2/3 antibody and the phosphorylation is diminished on treatment with lambda phosphatase. The experiments were performed in triplicate (*p < 0.05).