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. 2014 Jun 12;12:166. doi: 10.1186/1479-5876-12-166

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Copyright © 2014 Sabnis et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The Nanopro 1000 is capable of detecting signal using low cell numbers in primary AML samples. Three independent primary AML samples (A47316, A52453 and A52707) were used to test the capability of the assay to detect signal. Antibodies tested were Total 4EBP1 (A), Total Akt 1/2/3 (B), Phospho-4EBP1 Thr 37/46 (C) and Phospho-4EBP1 Ser 65 (D) Cell numbers ranged from 10,000-313 cells per capillary. Signal intensity (AUC, log-scale, y-axis) was plotted against cell numbers (log-scale, x-axis) and showed a linear increase with increasing cell numbers.