Figure 5.

Histopathological analysis of vascularity, inflammatory cell infiltration, and fibrosis. Tissue sections of “hyper-flow”, “hypo-flow”, and controls were stained with anti-factor VIII antibody, anti-CD163, CD3, CD79a MPO, or Masson’s trichrome. Positive-staining cells or areas were calculated using TissueMorph™. White, gray, and black bars represent control, “hypo-flow”, and “hyper-flow”, respectively. *p < 0.05. (A) The vascular walls were stained using an anti-factor VIII antibody, and the numbers of factor VIII-positive cells were calculated. (B) Inflammatory cell infiltrations were stained using anti-CD163, CD3, CD79a, and MPO antibody, and the numbers of positive-staining cells were calculated. (C) Fibrotic areas stained using Masson’s trichrome were evaluated, and the corresponding area was quantitated.