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. 2014 Jun 5;13:81. doi: 10.1186/1475-2859-13-81

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Copyright © 2014 Schmitz et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Discontinuous feed of DHEA to B. megaterium ATCC13368.B. megaterium ATCC13368 cultures were incubated at 30°C and 150 rpm in broth. After 16 h cultivation 400 μM DHEA was added (0 h) 5 times with 3 hours break between the individual additions. After the last addition (reaching 2000 μM DHEA in total) the cultures were incubated for additional 12 h. DHEA concentration in the cultures was calculated using TLC analysis and the software ImageLab™ (BIO-RAD, Germany).