A–F, EBA staining within the PVN, NTS and RVLM of WKYs (A–C; n=5) and SHRs (D–E; n=5). G and H, EBA immunostainning (red) and isolectin IB4 (H) within the PVN of a WKYs. I, merged figure showing co-localization (yellow). J, mean EBA immunoreactivity density in WKY, SHR and SHR-LOS (n=3).***P<0.0001 vs. WKY and +++P<0.0001 vs. SHR. Scale bar: 50µm. 3V: third ventricle.