Figure 3.
Expression of ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL-1) is specific to myositis muscle and does not occur in other types of dystrophic muscle. A, Quadriceps muscle lysates from 4 H or T mice and from 4 HT mice were obtained, and homogenates were immunoblotted for ubiquitin. B, Another set of quadriceps muscle lysates was obtained from 16-week-old H or T mice, HT mice, and age- and sex-matched SJL/J and mdx mice (n = 3 per group). The homogenates were immunoblotted with UCHL-1 or with β-actin as a loading control. C and D, The ratios of the respective proteins to β-actin from the experiments described in A and B, respectively, were calculated. Values are the mean ± SEM. *** = P < 0.001 versus other groups, by Student's t-test (C) or one-way analysis of variance (D). See Figure 1 for other definitions.