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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Cell Rep. 2014 Feb 27;6(5):818–826. doi: 10.1016/j.celrep.2014.01.045

Figure 2. Loss of Pten induces Fgf10 protein but not mRNA expression.

Figure 2

(A) qPCR analysis of Fgf mRNA expressions in the mouse epidermis. (B) Immunoblots of PtenCKO mice facial and cheek epidermis showed a reduction in Pten levels and up-regulation of pAKT, p4EBP1 and pS6. Although there were little changes in Fgf1, 2 and 7 expression, Fgf10 was strongly up regulated in PtenCKO mice. (C) Pten depleted keratinocytes switched from low to high Ca2+ media (basal to suprabasal state) showed increased levels of pAkt, p4EBP1, pS6 and Fgf10. (D-K) Immunohistochemistry confirmed strong pAKT, pS6, p4EBP1 and Fgf10 staining in the PtenCKO facial skin. (L) siRNA mediated PTEN depletion resulted in significant increase of pAKT, pS6 and FGF10 levels in human primary keratinocytes. (M-S) Both well-to-moderately and poorly differentiated human SCC stained weakly for PTEN but strongly for FGF10 in the epidermal layer. Arrows point to epidermis. Data are presented as mean ± s.e.m.