FIG. 2.
NOD.H2h4 thyrocytes are highly sensitive to iodide-induced apoptosis. Day 9 NOD.H2h4 and CBA/J thyrocyte cultures were exposed to the indicated final NaI concentrations for 24 h. Total (early and late) apoptotic cells were microscopically viewed and counted as described in Figure 1, using the FAM-FLICA assay. Three hundred cells were examined per group, and data represent percent apoptosis mean±standard error of the mean of two independent experiments. The percentage of necrotic cells in all cultures never exceeded 3.4%. Statistical significance was determined by a two-tailed unpaired t-test. *p=0.051; **p=0.025.