Mouse dorsal skin was exposed topically to either 200 μl acetone (VC) or 4 mg CEES (CEES), or treated with subcutaneous AEOL 10150 (SC) or subcutaneous + topical AEOL 10150 (TOF+SC) 1 h after CEES exposure. After 15 min, 1, 2 and 4 h of exposure or treatments, skin samples were collected and pharmacokinetic study to measure AEOL 10150 levels in the skin was carried out using HPLC as detailed under Materials and Methods. Data shown are mean ± SEM of 3 independent samples from each group. *, p<0.05 as compared to vehicle control group.