Table 1.

qPCR primers

Gene		Primer sequence	Primer concentration	Annealing temperature
Collagen I α1	Forward	AAGACATCCCACCAGTCACC	10 μm	60°C
	Reverse	CAGATCACGTCATCGCACA
Collagen I α2	Forward	GGCTCAACCTGAAGACATCC	4 μm	59°C
	Reverse	TCTCCTACCCAGACATGCTTC
Collagen III	Forward	CTGCTGGAAAGAATGGTGAG	10 μm	59°C
	Reverse	GTCACCAGAAGGCCCAGTA
Elastase 2	Forward	AGACTCCTTTGCCTCTGTCG	10 μm	58°C
	Reverse	AGCCTTTCTAGTGGGTCCTG
Tropoelastin	Forward	ATCTCTCAGTCAGGCACCAG	10 μm	58°C
	Reverse	GTTTGTTGGGAAAGAAAGCA
TGF-β1	Forward	GCTGACCCACAGAGAGGAAA	10 μm	60°C
	Reverse	AACTGAACCCGTTGATGTCC
eNOS	Forward	GATCAGCAACGCTATCACGA	10 μm	60°C
	Reverse	ATACGGCTTGTCACCTCCTG
18S rRNA	Forward	GTCTGTGATGCCCTTAGATGTC	10 μm	58–60°C
	Reverse	AAGCTTATGACCCGCACTTAC
Ribosomal protein S29	Forward	CAGGGTTCTCGCTCTTGC	10 μm	60°C
	Reverse	ACTGGCGGCACATATTGAG
beta-actin	Forward	TGTTACCAACTGGGACGACA	10 μm	60°C
	Reverse	GGGGTGTTGAAGGTCTCAAA

Forward and reverse primer sequences (5′–3′) used for qPCR to amplify collagen Iα1, collagen Iα2, collagen III, elastase 2, tropoelastin, transforming growth factor beta 1 (TGFβ1), endothelial nitric oxide synthase (eNOS), 18S rRNA, ribosomal protein S29 and beta-actin.